Coding

Part:BBa_K4759046:Design

Designed by: zhao shiyue   Group: iGEM23_Jiangnan-China   (2023-10-05)


fdr_0978-RBS2-fdx_1499-linker-GFP1-10


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1219
    Illegal BglII site found at 1037
    Illegal BglII site found at 2208
    Illegal BamHI site found at 1213
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1219
    Illegal PstI site found at 281
    Illegal PstI site found at 607
    Illegal PstI site found at 922
    Illegal NgoMIV site found at 625
    Illegal AgeI site found at 697
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 1342


Design Notes

Connecting GFP1-10 to the C terminus of fdx_1499 by linker and GFP11 to OleP, and the interacting protein will bring GFP11 to GFP1-10 close to the fluorescence, the stronger the interaction, we screened the two proteins with the strongest interaction force by bimolecular fluorescence.


Source

Ferredoxin reductase

References